Coding

Part:BBa_K883000:Design

Designed by: Jeroen Bosman   Group: iGEM12_Wageningen_UR   (2012-09-23)

CCMV wt coat protein coding


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

prefix – start codon - CCMV forward primer

5’-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGTACAGTCGGAACAGG-3’

suffix – CCMV reverse primer

5’-GTTTCTTCCTGCAGCGGCCGCTACTAGTACTTCACTAATACACCGGAGTGAAA-3’

Source

this part's source is an pET28 vector provided by Dr. Kormelink from the Virology group of the Wageningen UR

References